初始pH对厌氧环境下污泥中抗生素抗性基因行为特征的影响

污水处理厂产生大量的剩余污泥中含有丰富的抗性基因,给环境带来了潜在风险。以城市污水处理厂的剩余污泥为研究对象,在不同初始p H(对照组、初始p H=3、5、7、9、11)下观察厌氧条件下,8种抗生素浓度以及四环素类抗性基因(tet A、tet G、tet L、tet M、tet O、tet Q、tet W、tet X)、磺胺类抗性基因(sul I、sul II)和Ⅰ类整合子(int I 1)的行为特征。研究结果显示,初始p H对抗生素的降解影响较小,污泥中总抗生素的平均去除率为42%。对照组及初始p H为3、5、7、9、11下的总四环素类抗性基因分别削减0.65 log、0.96 log、0.75 log、0.62 log、0.86 log和0.98 log。不同四环素类抗性基因表现相似,在初始p H=3和初始p H=11下部分抗性基因削减较多,特别是tet A、tet G、tet L、tet O和tet X。2种磺胺类抗性基因均无削减,浓度平均上升0.18log。相关性分析显示,总抗性基因与TN、NH3-N、TP、SCOD(溶解性COD)均存在显著相关性(P0.05)。上述研究结果为污泥厌氧消化中抗生素抗性基因减量条件提供参考依据。     

超高压工程电缆绝缘油对海洋生物的生态毒性效应

随着超高压联网工程建设项目不断增多,取得巨大经济效益的同时,其电缆安全性日益受到关注。采用标准实验方法,研究电缆绝缘油对8种占据不同生态位的海洋生物(费氏弧菌(Vibrio fischeri)、牟氏角毛藻(Chaetoceros mueleri)、卤虫(Artemia sp.)、蒙古裸腹溞(Moina mongolica)、凡纳滨对虾(Litopenaeus vannamei)、裸项栉鰕虎鱼(Ctenogobius gymnauchen)、双齿围沙蚕(Perinereis aibuhitensis)、菲律宾蛤仔(Ruditapes philippinarum))的急性毒性,同时进行了电缆绝缘油对裸项栉鰕虎鱼14 d延长毒性实验和电缆绝缘油对蒙古裸腹溞的慢性毒性。结果表明,电缆绝缘油在50%饱和溶解浓度下未对费氏弧菌产生明显的发光抑制作用;对牟氏角毛藻、凡纳滨对虾、裸项栉鰕虎鱼、双齿围沙蚕和菲律宾蛤仔等5种生物未表现出急性毒性影响,其LC50均大于饱和浓度;对卤虫的96 h-LC50为17.07%,LOEC为12.5%,NOEC为6.25%;对蒙古裸腹溞96 h-LC50为29.75%,LOEC为25.0%,NOEC为12.5%;电缆绝缘油对卤虫和蒙古裸腹溞有剧毒。对裸项栉鰕虎鱼成鱼14 d延长毒性LC50大于500 000 mg·L~(-1);对蒙古裸腹溞母溞存活数的NOEC为625μg·L~(-1),LOEC为1 250μg·L~(-1);对母溞存活期的NOEC为312μg·L~(-1),LOEC为625μg·L~(-1);对产胎数的NOEC为625μg·L~(-1),LOEC为1 250μg·L~(-1);对产幼溞数的NOEC为625μg·L~(-1),LOEC为1 250μg·L~(-1)。     

Quantitative analysis of riparian herbaceous community in Liaohe River Conservation Area and its tributaries北大核心CSCD

Research on community characteristics of riparian herbs is an important scientific basis of riparian vegetation rehabilitation. This study aimed to investigate the species diversity and quantitative characteristics of riparian herbs in Liaohe River Conservation Area and its tributaries. Herbaceous communities were investigated by sample line method. Group average clustering analysis and detrended correspondence analysis (DCA) were used to find the major environmental factors affecting the distribution pattern of riparian herbaceous communities. The result showed altogether 154 herbaceous species, belonging to 40 families and 96 genera. The riparian herbaceous species diversity was found to be correlated to river slope, sinuosity and stream order, being lower in rivers of mountain area than in rivers of plain area, and higher in tributaries than in Liaohe River Conservation Area. Clustering analysis classified the herbaceous communities into 23 types, being dominated by hygrophytes including communities of Echinochloa crusgalli, Oenanthe javanica, Polygonum hydropiper, Murdannia keisak, Artemisia selengensis, Scirpus triqueter, Heleocharis soloniensis, Pycreus sanguinolentus, Cyperus fuscus, Phragmites australis, Polygonum amphibium, Carex diandra + Artemisia selengensis and Carex diandra + Rorippa islandica. Correlation analysis between DCA ordination axes and environmental factors showed that the altitude, river slope, sinuosity and stream level were the major environmental factors affecting the distribution pattern of herbaceous communities in the riparian zone of Liaohe River Conservation Area and its tributaries. The results of CCA showed that the contribution ratio of stream level was the highest, followed by altitude and slope, with sinuosity the last. The results suggested that riparian herbaceous characteristics are affected by the comprehensive force of altitude, river slope, sinuosity and stream order, and that Calamagrostis epigeios, Triarrhena sacchariflora and Phragmites australis are suitable species for riparian vegetation rehabilitation.     

Effects of initial decomposing leaf litter of Cinnamomum camphora on the growth and physiology of Impatiens balsamina北大核心CSCD

A pot experiment was conducted to study the allelopathic effects of initial decomposing leaf litter of Cinnamomum camphora on growth and physiology of Impatiens balsamina. Three leaf litter treatments included 20, 40 and 80 g of C. camphora leaf litter mixed with 8 kg of soil, namely T1, T2, and T3, respectively. In order to test the effect of leaf litter addition on the permeability and ventilation of soil simultaneously, a parallel trial with steamed leaf litter was conducted with the three treatments of the leaf litter. The leaf litter was steamed for 2 d to remove the secondary metabolites as much as possible, dried, and then mixed with 8 kg of soil, namely Z1, Z2, and Z3, respectively. No leaf litter was added in control (CK). The growth parameters of I. balsamina were determined at the 20 d, 60 d, 100 d and 120 d after sowing and the main physiology indicators were determined at the 60 d. The results indicated that: (1) The ground surface diameter and height of I. balsamina were inhibited significantly at 60 d (P < 0.05). Photosynthetic pigments and gas exchange parameters of I. balsamina were inhibited significantly at 60 d, and the inhibition effect was stronger with increased amount of leaf litter addition. The chlorophyll content, Pn and Ls decreased significantly with increased amount of leaf litter (P < 0.05). The activity of superoxide dismutase (SOD) and peroxidase (POD) in leaves of I. balsamina decreased with the increase of leaf litter addition. The content of MDA in treatments T1, T2 and T3 were all higher than that in CK, which indicated that I. balsamina suffered oxidative damage in a certain degree. The content of free proline (Pro) and soluble sugar (SS) in leaves of I. balsamina decreased significantly with the increase of the leaf litter (P < 0.05), while the content of soluble protein (SP) increased. (2) In the parallel trial, 60 d after sowing, no obvious difference was observed between CK and any steamed leaf litter treatment in terms of the morphological and physiological features stated above (P > 0.05). It indicated that the soil physical properties were not greatly influenced by leaf litter addition in the dose interval designed, or that the release of secondary metabolites from decomposing leaf litter was probably a better reason to explain the inhibition of leaf litter treatment to I. balsamina growth. (3)The compound effect (CE) of leaf litter decomposition on I. balsamina was enhanced with increase of the leaf litter, to 0.169, 0.354, and 0.497, respectively, in treatments of T1, T2 and T3. The study indicated that initial decomposition of C. camphora leaf litter in soil reduces the content of photosynthetic pigments, inhibits photosynthetic capacity and resistance physiology of I. balsamina, weakens its adaptability to the environment, and restrains growth of the plant. © 2015, Science Press. All rights reserved.     

基于多目标规划的防灾避难场所选址模型研究

给出了固定避难场所选址的影响因素,应用层次分析法与信息熵相结合的方法确定影响因素权重。建立了多目标规划选址模型,并用粒子群算法对模型进行求解。模型综合考虑了避难场所的灾害风险、区位规模和防灾救灾设施等因素,因此模型具有较高的实际应用价值。最后,结合实例,验证了该模型与求解算法的有效性和适用性。     

汶川地震极震区泥石流动力特征及参数研究——以安县高川乡为例

2008年汶川8.0级地震发生以来,极震区泥石流爆发频率有增无减且规模越来越大,破坏性越来越强,严重威胁灾区人民生命财产安全。对比采取现场勘查、遥感图像分析、历史资料对比分析等方法,分析了极震区泥石流动力特征受地形、地层、物源等因素的影响,特别是泥石流爆发过程中深侵蚀和溃决对泥石流动力特征的影响。针对四川安县高川乡流域泥石流沟的调查和分析,建立泥石流流量-冲刷模型,分析了常规地区泥石流峰值流量与极震区泥石流峰值流量差别,推导出极震区峰值流量及冲刷深度定量计算公式。通过实例分析,理论与实际具有较好的一致性,对极震区泥石流动力参数计算具有较好适用性和有效性。